Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts

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Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts.

A total of 393 clinical bacterial isolates were tested for their ability to agglutinate yeast cells of either Saccharomyces cerevisiae or Candida albicans. A positive agglutination of yeasts that could be prevented by methyl alpha-D-mannoside was taken as an indication for the possible presence of a mannose-specific lectin (carbohydrate-binding protein) on the surface of the tested bacteria. Ag...

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A lectin from the hemolymph of the banana shrimp Penaeus (Fenneropenaeus) merguiensis expressed higher agglutination activity against rabbit erythrocytes than those from human, and its activity was Cadependent. The hemagglutinating activity of the hemolymph lectin was stable up to 55 C and optimal at pH 7.5-8.0. N-acetylated sugars, ManNAc, GlcNAc, GalNAc, and NeuNAc, were effective inhibitors ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1980

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.11.4.328-331.1980